Determining viral specificity.

PRACTICAL4

Introduction

The purpose of this experiment is to demonstrate viral specificity. In this applicatory viral specificity is used as a tool to encounter the unknown bacteria. Known bacterio bacteriophages undersurface be used to date unknown bacteriophages by observing whether the bacteria argon lysed or not.

DISCUSSION

There strains that leave behind be affected by the bacteriophages and there are those that will not be affected. Those that lack the viral sense organ will show no reduction of the growth in the circulated regulate. The amount of reduction of growth in sensitive strains will depend on the viral specificity of the bacteriophage particles in the phage streak and on whether the virus is virulent or temperate. irate phages will show little or a big zone of inhibition where the phage drops has been inoculated.

Many bacteriophages are temperate and can establish a latent infection of their host termed lysogeny. In this state, the viral genome is known as a prophage, and the host is tell to be lysogenized or a lysogen.

Summary of procedure

Two phages of E .coli are inoculated in two separate test metros containing sterile PBS, and the test supply are named one and two respectively. Then intravenous feeding plates inoculated with four different unknown bacteria are named 1-4 respectively. Then individually plate is divided into two halves using a soft touch pen.



On each half, a circle is drawn and where the circle is drawn, a drop of PBS phage solution is placed. A circle where a drop is placed is where a viable zone of inhibition will occur depending on the metier of the phage solution.

Results obtained

Bacteria Test tube 1          Test tube 2

1          No clear zone          No clear zone

2          No clear zone          No clear zone

3          Clear zone          No...

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